AÇÃO DE Persea americana Mill. EM MODELO EXPERIMENTAL DE OSTEOARTRITE EM RATOS.

Abstract

Osteoarthritis is a worldwide degenerative chronic disease. Approximately 250 million people perform worldwide. Thus, the new forms of treatment are the disease of fundamental importance. The objective of this study was to evaluate the hydroethanolic extraction of leaves of Persea americana Mill in an experimental model of osteoarthritis in rat knees. Chemical characterization of the extract using phytochemical screening and high efficiency HLPC-UV array ethnography at 254 nm, identification by Mass Spectrometry Coupled Liquid Chromatography (LC-MS) and fluid flow analysis (FIAMS). Use and control of Wistar male males divided into two groups: EHPA (500mg / kg), saline (0.1mL) / kg), meloxicam (0.1mg / kg) and control subgroup that had no intervention.) For evaluation for motor activity the forced ambulation test was used. This was to quantify the frequency the Von-Frey test was used. A response to mechanical hyperalgesia was evaluated by the Randall Selitto test. Biochemical markers such as fasting glucose, total cholesterol, triglycerides, creatinine, urea, gamma GT, oxalacetic transaminase (AST) and pyruvic transaminase (ALT) were performed. They were also histological retrospectives of the knee joint using the OARSI criteria and radiographic analysis following the Kellgren and Lawrence osteoarthritis grade parameters. Qualitative phytochemical screening shows the presence of phenolic compounds, alkaloids, triterpenoid steroids, tannins and saponins. A chemical characterization by LC-MS and FIA-MSH. A presence of condensed tannins as major components, as well as phenolic acids, aglycone, glycosylated and triterpene flavonoids. Oral administration of the extract at a dose of 500mg / kg improved the dose of 500mg / kg orally and improved antinociceptive activity in the mechanical hyperalgesia test in both experiments. The extract did not alter liver, renal and hematopoietic functions. The histological and radiological analyzes that were extracted were a protector of cartilage in the combat process. Concluding the hydroethanolic extract of Persea americana Mill. reduced sensitivity to blood damage to adverse effects of meloxicam, a drug commonly used in medical practice.