EFEITOS DO ÓLEO DE COPAÍBA NO CONTROLE DA DOR VISCERAL E SEU EFEITO ANTI-INFLAMATÓRIO EM MODELO EXPERIMENTAL DE CISTITE

Abstract

Introduction: Interstitial cystitis (IC) is characterized by pelvic pain and chronic inflammatory process, irritative urinary symptoms for more than 6 weeks and has low efficacy with current treatments. The technical and ethical difficulty in studying in humans led to the development of experimental models to evaluate possible new treatments. Copaiba oil (OC) is known for its anti-inflammatory properties and has been traditionally used to treat inflammation and pain; however, its potential applicability in IC is unknown. Objective: To evaluate the anti-inflammatory and analgesic effects of copaiba oil in a rat model of cystitis. Methods: Sixty rats were divided into six groups: healthy saline (without IC induction and treated with 0.9% saline), induced saline (IC induction and treated with 0.9% saline) and meloxicam (positive control) and three groups treated with different concentrations of copaiba oil (50mg/kg, 100mg/kg and 200mg/kg). The cystitis model was induced by intraperitoneal administration of cyclophosphamide. The inflammatory response was evaluated by absolute mast cell count and histological evaluation of the bladder. Mechanical allodynia was measured by the Von Frey test, while pain was evaluated by the Grimace scale. Statistical analysis was performed by univariate analysis of variance (One-way ANOVA), followed by Tukey's test. Results: The groups treated with OC showed a positive response, with a progressive reduction in histological changes as the dose increased. In the 200mg/kg group, an almost complete normalization of the samples was observed, with slight circulatory congestion, without significant inflammatory infiltration (p 0.0327 in relation to the induced saline). All groups treated with OC showed a significant reduction in the mast cell count compared to the induced saline group, especially at the concentration of 200mg/kg (p: 0.007). In the Von Frey test, the groups treated with OC did not obtain statistically significant results, however, with a tendency of reduction compared to the induced saline group in the 200mg/kg group (p: 0.068). In the Grimace scale, the results were more disparate and did not present statistical significance compared to the induced saline (p: 0.571) but with a tendency of stabilization of pain from D7 to D14 in the 200mg/kg group. Conclusion: The study concludes that copaiba oil has a relevant anti-inflammatory effect and a probable analgesic effect.