Ivestigação do processo de polarização em macrófagos m1 e m2 durante infecção por Leishmania amazonensis

Abstract

Phagocytes are fundamental cells in the types of pixels and the pixels can be highlighted for the macrophages, they are characterized by the high plasticity of that allows a fast polarization for different profiles, with different functions. A literature on parasite species infection, an example of Leishmania sp., Is capable of inducing a polarization of M1 macrophages into M2 with consequent worsening of infection. Thus, the present work aims to investigate a polarization of M1 or M2 macrophages during infection by Leishmania amazonensis. Assays of the non-stimulated RAW 264.7 macrophages (M0), polarized for M1 and M2 were infected with L. amazonensis at the ratio of 10 parasites to 1 macrophage for 24 h. Afterwards, the infection rate, nitric oxide (NO) dosage, cytokine quantification, hydrogen peroxide production (H2O2) were analyzed. In the report of inhibition of iNOS was carried out by the use of the drug aminoguanidine, while a route of the amastigotes was validated by labeling via Annexin V / Propidium Iodide. Expression of iNOS, CD80 / 86 and F4 / 80 were found by immunophenotyping. Our data were recorded as a parasite rate for M1 macrophages, and they were increased in NO production, these data are correlated for this group. Macrophages when treated with aminoguanidine have been reduced in infection rate for all groups. M 1 and the low production of hydrogen peroxide, unlike macrophages M0 and M2. The viability data of the amastigotes demonstrated that there were higher percentages of apoptosis of parasites recovered from macrophages throughout the treatment with iNOS inhibitor. Inflammatory cytokines (IL-6, TNF-α) and an MCP-1 chemokine were also shown to be increased in M1 macrophages, as well as overexpression of iNOS, CD86 / 80 and F4 / 80 present in cells when infected by parasite Based on data, we can conclude that L. amazonensis infection in M1 macrophages has been shown to potentiate a production of mediators related to the characterization of this phenotype, where it is worth mentioning nitric oxide, which deleterious age during a response to parasite culminating without worsening of the infection. Key words: