Análise da expressão proteica da P53 em adenomas hipofisários

Abstract

The pituitary adenomas represent about 10 to 15% of intracranial tumors and are usually benign, comprising a cell population of monoclonal origin. They are classified as macroscopic and radiological aspects, functional status, dyeing, immunohistochemistry and microscopic. About 70% of human cancers have mainly a deficiency in the function gene. The p53 gene is extensively studied in tumors, showing that patients with mutations have a worse prognosis, as well, the p53 protein is critical in preventing tumor development performing change detection function in DNA and thereby fix or apoptosis. It is possible that the increase in expression of this protein is the result of an attempt to stop the cell cycle in response to deregulation by a stimulus from another source. This way, the study seeks to determine the expression of p53 protein in pituitary adenomas. an analytical study, with crosssectional was held in which were included 62 patients older than 16 years of both sexes, who were diagnosed with pituitary adenoma, from 2008 to 2016, arising from the Endocrinology Service University hospital of the Federal University of Maranhão - HUUFMA. These patients underwent surgical resection of the tumor, obtaining the samples of tumor tissue. After conventional histopathology, they were embedded in paraffin, for carrying out the immuno-histoqumico study aimed to identify the expression of p53 protein and implications correlated with biological behavior of pituitary adenomas. The analysis for p53 protein expression in adenomas was obtained by immunoenzymatic of streptoavidin-biotin or immunohistochemistry. The p53 protein immunostaining revealed no statistically significant correlation, as the clinical and demographic characteristics such as gender, age, p53 expression in tumor subtypes and tumor volume. It is necessary to obtain greater numbers of patients, so that p53 expression is effective when the real prognostic value in pituitary adenomas.